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Chapter 3 | QuantiGene Plex DNA Assay Procedure 17
Washing QuantiGene Plex Assay Plates with BioTek ELx 405 Magnetic Plate Washer
To wash plates:
1. Turn on the plate washer.
2. Run a RINSE followed by a PRIME_200 program with distilled water in Buffer B container.
A. To RINSE, go to Main Menu >Run > Maint >Day_Rinse >Rinse.
B. To PRIME, go to Main Menu >Run >Prime >Prime_200 (default set by the manufacturer).
3. Place QuantiGene Wash Buffer in Buffer A container.
4. Run another PRIME_200 program.
5. Select the QuantiGene Plex wash program.
Go to Main Menu >More >LINK 01 (as defined in Creating a Wash Program on page 16)
6. Place Magnetic Separation Plate on top of the plate holder and press Start.
7. When switching to SAPE Wash Buffer, place it in Buffer A container and repeat steps 4-5.
8. When finished washing, place distilled water in Buffer A container and run the PRIME_200 program
3 times.