Download GeneQuant pro User Manual

Transcript
CONTENTS
Unpacking, Positioning and Installation
Essential Safety Notes
Rear panel
OPERATION
Introduction
Instrument Description
Utility keys
Reading keys
4
5
5
6
6
7
7
8
TECHNIQUES
DNA, RNA and oligonucleotide characterisation
Nucleic Acid Quantification (NAQ)
Nucleic Acid Purity Checks
Use of Background Correction
Protein Determination
Protein Determination at 595, 546 and 562 nm
Protein Determination at 280 nm
Bacterial Cell Culture Measurement
9
9
9
10
11
12
12
12
13
SET UP AND SAMPLE MEASUREMENT
Set up
Sample measurement
DNA, RNA and oligo
Base Sequence
Tm
Protein 595 Assay
Protein 280 Measurement
Bacterial Cell Culture
14
14
14
16
18
19
20
22
22
MESSAGES
23
OUTPUT OF RESULTS
Use with parallel printer
Use with PC
24
24
24
CELLS
Selecting the appropriate cell
Comparison of instrument with other spectrophotometers
Filling cells
Taking measurements
Cleaning cells
Reproducibility using reduced aperture ultramicrovolume and capillary cells
25
25
26
26
26
27
27
___________________________________________________________________
Issue 06 - 12/2003
GeneQuant pro, English
2