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CONTENTS Unpacking, Positioning and Installation Essential Safety Notes Rear panel OPERATION Introduction Instrument Description Utility keys Reading keys 4 5 5 6 6 7 7 8 TECHNIQUES DNA, RNA and oligonucleotide characterisation Nucleic Acid Quantification (NAQ) Nucleic Acid Purity Checks Use of Background Correction Protein Determination Protein Determination at 595, 546 and 562 nm Protein Determination at 280 nm Bacterial Cell Culture Measurement 9 9 9 10 11 12 12 12 13 SET UP AND SAMPLE MEASUREMENT Set up Sample measurement DNA, RNA and oligo Base Sequence Tm Protein 595 Assay Protein 280 Measurement Bacterial Cell Culture 14 14 14 16 18 19 20 22 22 MESSAGES 23 OUTPUT OF RESULTS Use with parallel printer Use with PC 24 24 24 CELLS Selecting the appropriate cell Comparison of instrument with other spectrophotometers Filling cells Taking measurements Cleaning cells Reproducibility using reduced aperture ultramicrovolume and capillary cells 25 25 26 26 26 27 27 ___________________________________________________________________ Issue 06 - 12/2003 GeneQuant pro, English 2