Download User manual AS110, edition 7
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14 User manual AS110, edition 7 A wash routine is performed after each injection. Air segment with Full loop injections An air segment of 5 µL (0.5 µL for micro version) can be used to minimize mixing of sample during aspiration. This air segment is at the front of the flush volume and will not be injected. With a standard 15 µL needle, the flush volumes must be a minimum of 30 µL + air segment (5 µL) and 30 µL for injections without air segment. With a 2.4 µL micro needle, the flush volumes must be a minimum of 4.8 µL + air segment (0.5 µL) and 4.8 µL for injections without air segment. If samples are highly viscous it may be necessary to program larger flush volumes and reduce the syringe speed for better performance.