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APPLIED OPERATIONS/Image Acquisition
1 Setting the Z-direction scanning range
While acquiring image, move the Z stage according to the range of the multiple sections
to be observed (Z-direction scanning range).
From the panel page tabs shown on the bottom right of the [Acquire] panel, select the [Z
Stage] sub-panel.
<Go> button
Moves to the set scanning stop position.
Use this button to check the scanning
stop position.
[Stop Z] text box
Shows the scan stop position in the
range of the observed cross-section (Zdirection scanning range).
<Set> button
Sets the current stage position as the
scanning stop position of the range of
the observed cross-section (Z-direction
scanning range).
<Z stage coarse adjustment> buttons
Displaces the Z stage on a large scale.
<Z stage fine adjustment> buttons
[Current Pos] text box
Displaces the Z stage on a fine
scale.
Shows the current position of the stage.
<Set Zero> button
[Start Z] text box
Sets the current stage position as the
home position. Pressing this button also
clears the [Stop Z] and [Start Z] values.
Shows the scan start position in the
range of the observed cross-section (Zdirection scanning range).
<Set> button
[Step Size] text box
Sets the current stage position as the
scanning start position of the range of
the observed cross-section (Z-direction
scanning range).
Set the number of steps using the < >
or < > button. This number can also
be input directly from the keyboard.
Recommended step size
<Go> button
Shows the number of steps calculated
by the system so that the scale of depth
in the Z-direction of the acquired image
is identical to the scale of the plane in
the X- and Y-directions.
Moves to the set scan start position.
Use this button to check the scan start
position.
[Slices] text box
Shows the number of images acquired.
This number can also be input directly
from the keyboard.
TIP
[Locked] check box
Fig. 2-18
[Z Stage] Sub-panel
Enables the Z-Motor operation.
The moving amount assigned to the <Z stage fine adjustment> and <Z
stage coarse adjustment> buttons can be changed.
See section 1-3 in MAINTENANCE, “Setting the System Configuration” for
detailed operations.
1.
Check the [Locked] check box in the [Z Stage] sub-panel.
Do not turn the fine focus adjustment knob while the [Locked] check
box is checked, for this may damage the Z motor.
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