Download LCquan 2.5.6 Quantitative Analysis User Guide

3 Exploring Your Data
Creating a Quan Component from the Peak List
Creating a Quan Component from the Peak List
This topic demonstrates how to create a quan component in the Explore view, and then
export it to the Quantitate view. Creating quan components is the first step in creating a
Processing Method.
The components that LCquan creates out of the peak list are based on the Explore Method
that you define in the Create Explore Methods page. In the previous topic, you set up a
method that’s a TIC (no filter). For steroids, this resulted in a chromatogram and peak list
with four peaks. In general, you want to filter first to get one peak in the peak list and then
generate its quan component. You then add quan components to the Processing Method one
at a time.
Y To create the deoxycorticosterone quan component
1. Click on the Method button to display the Create Explore Methods page.
2. Enter or select the following Explore Method settings:
• Detector: MS
• Trace: Mass Range
• Masses: 109.0, 253.1, 295.2
• Smoothing: 7
• Peak Detection Algorithm: Genesis
• Filter: + c Full ms2 331.30@40 [100.00-350.00]
3. Change the bottom-right pane to Peak List.
4. Click on Apply to apply the settings. See Figure 29.
5. Select Peak Name: 1.383 and type in deoxycorticosterone to rename the peak. See
Figure 30.
Figure 29. Initial peak list that was obtained with the Explore Method of step 2
Thermo Scientific
LCquan User Guide
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