Download RTPCR User Guide - Gene

User Guide
QPCR
Table of content
Table of content....................................................................................................................... 2
1
Introduction ..................................................................................................................... 4
1.1
Purpose.................................................................................................................... 4
1.2
Hints for using the software .................................................................................... 4
1.3
Example of a standard workflow ............................................................................ 4
2
General information ........................................................................................................ 6
2.1
Welcome screen ...................................................................................................... 6
2.2
Header section......................................................................................................... 7
2.3
Navigation section................................................................................................... 9
2.4
Information section ................................................................................................. 9
2.5
Symbols................................................................................................................. 12
3
Upload and Parse........................................................................................................... 13
3.1
File Upload............................................................................................................ 13
3.2
Multiple Upload .................................................................................................... 15
3.3
Multiple Parse ....................................................................................................... 16
4
Run ................................................................................................................................ 17
4.1
Create a Run.......................................................................................................... 17
4.2
Run List................................................................................................................. 18
4.3
Display Run........................................................................................................... 18
4.4
Parse Run .............................................................................................................. 20
4.5
Parser Log ............................................................................................................. 21
4.6
Progress Information............................................................................................. 23
5
Plate............................................................................................................................... 24
5.1
Well ....................................................................................................................... 25
5.2
Charts .................................................................................................................... 26
5.3
Cq Analyze Results ............................................................................................... 28
6
Experiment .................................................................................................................... 30
7
Project ........................................................................................................................... 32
8
Analyze ......................................................................................................................... 33
8.1
Analyze Setup ....................................................................................................... 33
8.2
Cq Analyze Log .................................................................................................... 37
8.3
Normalization Result ............................................................................................ 38
8.4
Normalization Result – Bars ................................................................................. 40
9
Statistical Test ............................................................................................................... 43
9.1
Test Setup.............................................................................................................. 43
9.2
Graphical Result.................................................................................................... 45
10
Error propagation ...................................................................................................... 47
11
User settings .............................................................................................................. 48
12
Run Deletion Log...................................................................................................... 49
13
PCR Management ..................................................................................................... 51
13.1 CDNA ................................................................................................................... 51
13.2 Target .................................................................................................................... 51
13.3 Instrument Setting ................................................................................................. 52
13.4 Passive Reference.................................................................................................. 54
13.5 Primer.................................................................................................................... 55
13.6 Primer Validation .................................................................................................. 55
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Stephan Pabinger