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7 Optimizing Your Method Modifying an Automatic Tune – 50—Select this option to measure the width at 50% of the peak height. • Threshold—Use this field to enter the change in intensity that has to occur when the tune to select a new voltage. For example, if you set this field to 1.1, the tune will not select a new voltage for that component, unless the intensity is 110% of the old intensity. If you set this field to 1, anytime the new voltage has an intensity larger than the old intensity, the tune will select the new intensity. Note If a value less than 1 is chosen for ramped devices, the tune will step back in energy until the lower intensity is detected. For example, if 0.8 is chosen, the device value lower in energy than the device value with the maximum response which gives an intensity closest to 80% of the maximum intensity will be chosen. • On Error—Use this pull-down menu to select how to handle an error in the tune. – Fail—Select this option to stop the tune when an error occurs or if no tune points are found meeting the tune criteria. – Continue—Select this option to allow the tune to continue on to the next device when an error occurs. 6. Click the Resolution tab to configure the resolution of your tune. You can tune the resolution during a lens tune or you can tune the resolution by itself. Note Click the Advanced Settings check box to access the Resolution tab. • Tune Resolution—Select this option to tune the resolution by itself. This resolution will tune the system at 100 and 1000 amu/s scan rates. You may also tune at a higher scan rate. 100 ISQ User Guide Thermo Scientific