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Sample Preparation (1 µg DNA Samples)
Step 3. Repair the ends
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Step 3. Repair the ends
Use the SureSelectXT2 Library Prep Kit.
CA U T I O N
This section contains two versions of instructions for the end repair reaction. The first
section covers kits supplied with Library Prep Kit p/n 5500-0130 or 5500-0131, with two
separate reagent vials containing the End Repair Enzyme Mix and End Repair Oligo Mix
(typically received February, 2015 or later).
The second section covers kits supplied with Library Prep Kit p/n 5500-0102 or
5500-0103, with pre-combined End Repair Master Mix (typically received before
February, 2015).
Verify that you are referencing the information appropriate for your kit version before
you proceed.
Hold samples on ice during the following steps.
Instructions for Library Prep Kit p/n 5500-0130 or 5500-0131
1 Prepare the appropriate volume of End Repair Reaction Mix, as
described in Table 7, on ice. Mix well on a vortex mixer.
Table 7
Preparation of SureSelect End Repair Reaction Mix
Reagent
Volume for 1
reaction
Volume for 16 reactions
(includes excess)
SureSelect End Repair Enzyme Mix
40 µL
660 µL
SureSelect End Repair Oligo Mix
10 µL
165 µL
Total
50 µL
825 µL
2 To each 50-µL sheared DNA sample well, add 50 µL of the SureSelect
End Repair Reaction Mix prepared in step 1.
3 Mix well by pipetting up and down or by gentle vortexing.
SureSelectXT2 Target Enrichment System for Illumina
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