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Section 2.3 S.U.M. BPC Loading Instructions Figure 2.32 6. 7. Once the bag is fully seated, check that the drain line remains fully extended through the bottom port before proceeding to the next step. To keep the drain line from contacting the floor, the line can be guided through one of the bars located on the base of the outer support container. Attach the impeller sleeve directly to the reusable bearing port via the quick connect attachment on the plastic sheath. An audible click will confirm that the attachment is made. (Figure 2.34). NOTE: For the 2000 L unit, make the tri-clamp connection (without a gasket) using a plastic clamp. Figure 2.34 8. Powder Addition with the Thermo Scientific HyClone Powdertainer II Thermo Scientific Figure 2.33 Figure 2.35 Insert the drive shaft into the motor as instructed in the previous section. The Single-Use Mixer can be equipped with a Powdertainer bag support stand for solids addition (see Section 4.3 for ordering information). The following instructions highlight the HyClone Powdertainer BPC in conjunction with the S.U.M. when equipped with the bag support stand and holding arm. Other methods for solids addition can also utilize the powder port. Complete protocols are not included here as individual protocols vary; however, general descriptions of these processes are Single-Use Mixer 37