Download - Bio-Rad

Transcript
Table of Contents
Introduction1
Principle2
Kit Contents and Storage
4
Recommended Materials
5
Assay Workflow
6
Important Considerations
7
Detailed Instructions
1. Plan Plate Layout8
2. Prepare Instrument9
3. Prepare Wash Method10
4. Prepare Standards11
5. Prepare Samples16
6. Prepare Coupled Beads20
7. Run Assay22
8. Read Plate
28
Troubleshooting Guide
35
Appendix: Protease Inhibitors
40
Plate Layout Template
41
Calculation Worksheet
42
Safety Considerations
46
Legal Notices
46
Ordering Information
47