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Table of Contents Introduction1 Principle2 Kit Contents and Storage 4 Recommended Materials 5 Assay Workflow 6 Important Considerations 7 Detailed Instructions 1. Plan Plate Layout8 2. Prepare Instrument9 3. Prepare Wash Method10 4. Prepare Standards11 5. Prepare Samples16 6. Prepare Coupled Beads20 7. Run Assay22 8. Read Plate 28 Troubleshooting Guide 35 Appendix: Protease Inhibitors 40 Plate Layout Template 41 Calculation Worksheet 42 Safety Considerations 46 Legal Notices 46 Ordering Information 47