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Figure 25: Variation of gradient magnitude along probe line Figure 26: Gradient of the magnitude result showing edge of objects in the image 3.5.3 Distance Map approach The distance operation is used for the particle segmentation before the watershed operation. It calculates the distance function of a binary image onto a gray level image, as the sum of successive erosions. In the output image O, a pixel x belonging to a particle X, takes an intensity I equal to the distance in pixel units to the boundary [Avizo, 2009]. The distxxx is command is used for the distance operation because it 34