Download Version 1.2.31 Release date, February 16, 2002
Transcript
GEArrayAnalyzer User Manual Table of contents 1 GETTING STARTED .......................................................................................................... 3 1.1 INTRODUCTION ................................................................................................................................ 3 1.2 SYSTEM REQUIREMENT ................................................................................................................... 3 1.3 INSTALLATION ................................................................................................................................. 3 1.4 BACKGROUND INFORMATION FOR USING THE SOFTWARE ................................................................ 4 1.4.1 Overview of data analysis....................................................................................................... 4 1.4.2 Recording your array image................................................................................................... 4 1.4.3 Converting the image file into the raw data file ..................................................................... 4 1.4.3 The Format of the Raw Data File ............................................................................................. 7 FOR USING RAW DATA TABLES IN GEARRAYANALYZER, PLEASE REFER TO INSTRUCTION IN SECTION 2.2.4, INPUT DATA FILES.................................................................... 8 2 USING GEARRAYANALYZER .................................................................................. 9 2.1 DATA PROCESSING OPTIONS ............................................................................................................ 9 2.1.1 Background subtraction.......................................................................................................... 9 2.1.2 Normalize to Positive Controls or to the median value of the experiment.............................. 9 2.1.3 Normalizing All Experiments to Specific Experiments ......................................................... 10 2.2 STEP-WISE GUIDE FOR DATA ANALYSIS ......................................................................................... 10 2.2.1 Opening window ................................................................................................................... 10 2.2.2 Import Gene List ................................................................................................................... 10 2.2.3 Saved analysis....................................................................................................................... 11 2.2.4 New analysis ......................................................................................................................... 11 2.2.5 Data Analysis Window.......................................................................................................... 13 3 GEARRAY DATA ANALYSIS FREQUENTLY ASKED QUESTIONS...................................................................................................................................... 16 2