Download Manual: ABI 173A MicroBlotter

Applied Biosystems
8. Program the 140D per the instructions listed under "System
Installation Test Program" on page 2-36.
Note
This must be done prior to step 12 below.
9. Prepare a solution of Dye Mark II by adding 38 µL of 0.1% TFA in DI
water to a clean Eppendorf tube. Then add 1 µL of each Dye Mark, and
vortex the solution for 30 sec.
10. Load the Dye Mark solution into the 25 µL syringe provided with this
system. Insert the needle of the syringe into the 112A.
11. Ensure the 112A is in load position.
12. Begin execution of the test program by pressing Run on the 140D. The
Run Select Screen (Figure 2-19) will be displayed.
PROG
1. 9
#RUNS
1
PROG
# RUNS
BEGIN>
4.
2.
5.
3.
6.
DELETE>
MANUAL? N
Figure 2-19. RUN SELECT Screen
13. Position the cursor at 1. under PROG. Enter 9 (the program number),
and 1 for #RUNS (number of runs).
14. Press the BEGIN> soft key to start the program. The syringes fill
automatically, and the RUN STATUS Screen (Figure 2-20) is displayed.
TIME:
FLOW
%B
PRESS
EVENTS:
PROG NO.
CAP A
CAP B
STEP NO.
RUN
Figure 2-20. RUN STATUS Screen
15. Watch the 140D during the prepressurization phase. The pump should
reach 800 psi in less than 1 min. If it does not, check the system for
leaks, or purge the 140D again as appropriate.
April 2002
2 Unpacking and Installation
2-33