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Relative Standard Curve Method for Quantification
Relative Standard Curve Method Results
Each c-mycN value in Table 3-14 on page 3-33 is divided by the brain c-mycN value
to give the values in the final column. These results indicate the kidney sample
contains 5.5 times as much c-myc mRNA as the brain sample, liver 34.2 times as
much, and lung 15.7 times as much.
Note: Averages and standard deviations are calculated from unrounded data, not the
rounded data presented here.
Table 3-14
Amounts of c-myc and GAPDH in human brain, kidney, liver, and lung tissues
c-myc
ng Total Raji RNA
GAPDH
ng Total Raji RNA
Brain
0.033
0.51
(Designated
calibrator)
0.043
0.56
0.036
0.59
0.043
0.53
0.039
0.51
0.040
0.52
Average
0.039±0.004
0.54±0.034
Kidney
0.40
0.96
0.41
1.06
0.41
1.05
0.39
1.07
0.42
1.06
0.43
0.96
0.41±0.016
1.02±0.052
0.67
0.29
0.66
0.28
0.70
0.28
0.76
0.29
0.70
0.26
0.68
0.27
0.70±0.036
0.28±0.013
Tissue
Average
Liver
Average
Sequence Detection Systems Chemistry Guide
c-mycN
Norm. to GAPDH a
c-mycN
Rel. to Brain b
0.07±0.008
1.0±0.12
0.40±0.025
5.5±0.35
2.49±0.173
34.2±2.37
DRAFT
November 13, 2003 12:42 pm, 3 QuantAssays.fm
3-33