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4. Create/Edit a Method Wizard Min./Max./Area Tab Use this tab to define the evaluation of the minimum and maximum values contained in the curve and to define the calculation of the area under the kinetic curve. The Min./Max./Area tab contains the following elements: Input data drop down list Select the input data to be processed. Mean minimum value check box Select to open the Points text field where the fixed number of points has to be entered for the minimum value. An even curve will then be generated using these points and the lowest value contained in the curve determined. Mean maximum value check box Select to open the Points text field where the fixed number of points must be entered for the maximum value. An even curve will then be generated using these points and the highest value contained in the curve determined. Area check box The start and end time can be typed into the provided text fields, otherwise the whole kinetic is analyzed. • Time/Points option buttons: If Time is selected the start and end time is entered in hours, minutes and seconds. If Points is selected the start and end point for the analysis is given through the entered kinetic cycle number. • Start field: The start time (in hours, minutes and seconds) or cycle number has to be entered here. • End field: The end time (in hours, minutes and seconds) or cycle number has to be entered here. Averages are taken from the number of points starting from the first kinetic measurement value and stepping through one by one until the last kinetic measurement point is included in the average calculation. The minimum/maximum value is determined from these averages. The result data made available through this method includes minimum/maximum value, and the time span from the first measurement to the minimum/maximum value in seconds. Example: With a kinetic of 5 cycles and a fixed number of 3 points, following averages are taken: st nd rd nd rd th rd th th average of 1 , 2 , and 3 kinetic value average of 2 , 3 , and 4 kinetic value average of 3 , 4 , and 5 kinetic value The minimum/maximum value is determined from these 3 averages. The area under the curve is calculated using the formula given below: n −1 A = ∑ y i * ( xi +1 − xi ) + i =1 2013-08 ( y i +1 − y i ) * ( xi +1 − xi ) 2 Instructions for Use for magellan No. 30066407 Rev No. 1.3 79