Download Test Waters

Procedure for making the buffer
Dissolve 1g Laureth 12 in 100 ml DI water using 150/250ml beaker by
gentle heating and transfer in to 1000-ml volumetric flask. Rinse the
beaker several times till shaking the water in the beaker generates no
foam. (Or add 10 ml of 10% Laureth 12 to 1000 ml volumetric flask
and rinse the pipette tip several times to transfer all the detergent.) Add
10 ml of 1.0 M Tris, p 7.4 and 2 ml of 0.5M EDTA, pH 8.0 and 150 l
of antifoam A if required. Make the volume to 1000 ml with DI water.
1 Stain Kit (BTF Precise Microbiology, Australia or Waterborne Inc,
USA) -Fluorescent antibody staining kit containing a) stain b) Wash
buffer c) Positive control and d) Mounting Medium
2 Millipore Membrane filters: 0.8 µm ATTP (47 mm) and 0.4 µm HTBP
(13 mm or 25 mm)