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ECM ® 2001 Electrofusion/Electroporation Systems
Operating Basics
(Continued)
Selection of Operating Mode
Automatic Start of the fusion process is initiated by pressing the
Automatic Start button (9); in this case, the pre-set AC Voltage and
AC Duration is applied. Alignment is followed automatically by electroporation settings of DC PULSE Voltage and Pulse Length.
Manual Start (Best with Microslides viewed under a microscope). Push
MANUAL START button (12) to turn on the alignment AC voltage. It
will illuminate in this position. By pushing it in a second time, the AC
alignment voltage is turned off and electroporation pulses are delivered
to the test chamber.
Alignment Parameters
AC Voltage: Dial the voltage setting required using the AC
Voltage(1).
AC Pulse Length: Dial the AC Pulse Length on thumbpot
AC Duration switch (2).
Electroporation Parameters
Choose between HV or LV Mode (4).
Pulse Length: Dial the pulse Length on DC Pulse Length
switch (5).
DC Voltage: Select the electroporation pulse Voltage by Set
Voltage (6).
Number of DC Pulses: Set Number of pulses (7).
Post Fusion AC Parameters
Set the Post Fusion AC (3) time for compression of cells after
electrofusion.
The post fusion AC amplitude is 1/10 of the alignment AC amplitude.
The post fusion AC amplitude attenuates with time.
Operation With Microslides & Microscope
To connect Microslides to the ECM® 2001 pulse generator, attach
Banana-to-Micrograbber Adapters to each HV cable and then connect
the micrograbbers to the microslide contacts. It is advisable to tape
the cables to the microscope stand to avoid the weight of the cables
moving the Microslide. The Microslide wire electrode gap can be filled
and emptied with a micropipette tip or a syringe with a hypodermic
needle.
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