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Part two FluorSep utility
You use the Find Fluors feature to place the fluorochrome marker rectangles
(section 2.4). Next, you recenter or resize the fluorochrome marker rectangles
(section 2.5). Finally, you place the background rectangles in the correct areas
of the background (section 2.6).
Figure 2-4. The rectangles that appear when you use the Manual background
correction method on an image containing four fluorochrome markers.
p2-6
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ImageQuant Utilities User’s Guide