Download UCSFChimera Workshop - The UCSF Computer Graphics Laboratory

Use Sequence dialog (Tools -> Structure Analysis -> Sequence) to select first alpha helix. Restrict
map and model to it. Make mesh transparent and yellow.
Show lysine sticking out. Hover mouse to identify residue.
Fit Alpha/Beta Tubulin Dimer into Electron Microscopy
Density Map
Show alpha and beta monomers as ribbons and color yellow and orange.
Move into density map, select all atoms, zone at 50 A radius (for faster rotation), make map
transparent, and optimize fit. Clear selection before inspecting fit (for faster rotation and clearer
view).
Can try fit shifted right by 1 monomer. Slightly different fit. Would be interesting to try fitting each
monomer separately.