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Chapter 10 Viewing and editing sequences Contents 10.1 View sequence . . . . . . . . . . . . . . . . . . . . . . . . 10.1.1 Sequence settings in Side Panel . . . . . . . . . . . . 10.1.2 Restriction sites in the Side Panel . . . . . . . . . . . 10.1.3 Selecting parts of the sequence . . . . . . . . . . . . 10.1.4 Editing the sequence . . . . . . . . . . . . . . . . . . 10.1.5 Sequence region types . . . . . . . . . . . . . . . . . 10.2 Circular DNA . . . . . . . . . . . . . . . . . . . . . . . . . . 10.2.1 Using split views to see details of the circular molecule 10.2.2 Mark molecule as circular and specify starting point . . 10.3 Working with annotations . . . . . . . . . . . . . . . . . . 10.3.1 Viewing annotations . . . . . . . . . . . . . . . . . . . 10.3.2 Adding annotations . . . . . . . . . . . . . . . . . . . 10.3.3 Edit annotations . . . . . . . . . . . . . . . . . . . . . 10.3.4 Removing annotations . . . . . . . . . . . . . . . . . . 10.4 Sequence information . . . . . . . . . . . . . . . . . . . . . 10.5 View as text . . . . . . . . . . . . . . . . . . . . . . . . . . 10.6 Creating a new sequence . . . . . . . . . . . . . . . . . . . 10.7 Sequence Lists . . . . . . . . . . . . . . . . . . . . . . . . 10.7.1 Graphical view of sequence lists . . . . . . . . . . . . 10.7.2 Sequence list table . . . . . . . . . . . . . . . . . . . 10.7.3 Extract sequences . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131 132 137 143 144 145 145 146 147 147 148 152 154 154 154 155 156 157 157 158 159 CLC Combined Workbench offers five different ways of viewing and editing single sequences as described in the first five sections of this chapter. Furthermore, this chapter also explains how to create a new sequence and how to assemble several sequences in a sequence list. 10.1 View sequence When you double-click a sequence in the Navigation Area, the sequence will open automatically, and you will see the nucleotides or amino acids. The zoom options described in section 3.3 allow 131