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CHAPTER 18. CLONING AND CUTTING
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Number of cut sites
Clicking Next confirms the list of enzymes which will be included in the analysis, and takes you
to the dialog shown in figure 18.43.
Figure 18.43: Selecting number of cut sites.
If you wish the output of the restriction map analysis only to include restriction enzymes which
cut the sequence a specific number of times, use the checkboxes in this dialog:
• No restriction site (0)
• One restriction site (1)
• Two restriction sites (2)
• Three restriction site (3)
• N restriction sites
Minimum
Maximum
• Any number of restriction sites > 0
The default setting is to include the enzymes which cut the sequence one or two times.
You can use the checkboxes to perform very specific searches for restriction sites: e.g. if you
wish to find enzymes which do not cut the sequence, or enzymes cutting exactly twice.
Output of restriction map analysis
Clicking next shows the dialog in figure 18.44.
This dialog lets you specify how the result of the restriction map analysis should be presented:
• Add restriction sites as annotations to sequence(s). This option makes it possible to see
the restriction sites on the sequence (see figure 18.45) and save the annotations for later
use.