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CHAPTER 17. SEQUENCING DATA ANALYSES AND ASSEMBLY
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• Scale traces. A slider which allows the user to scale the height of the trace area. Scaling
the traces individually is described in section 17.1.1.
Figure 17.3: A sequence with trace data. The preferences for viewing the trace are shown in the
Side Panel.
17.2
Multiplexing
When you do batch sequencing of different samples, you can use multiplexing techniques to
run different samples in the same run. There is often a data analysis challenge to separate
the sequencing reads, so that the reads from one sample are mapped together. The CLC DNA
Workbench supports automatic grouping of samples for two multiplexing techniques:
• By name. This supports grouping of reads based on their name.
• By sequence tag. This supports grouping of reads based on information within the
sequence (tagged sequences).
The details of these two functionalities are described below.
17.2.1
Sort sequences by name
With this functionality you will be able to group sequencing reads based on their file name. A
typical example would be that you have a list of files named like this:
...
A02__Asp_F_016_2007-01-10
A02__Asp_R_016_2007-01-10
A02__Gln_F_016_2007-01-11
A02__Gln_R_016_2007-01-11
A03__Asp_F_031_2007-01-10