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RayBiotech, Inc. RayBio® Mouse Cytokine Antibody Array G Series 6 Protocol TABLE OF CONTENTS I. Introduction……..……………………………... 2 How It Works………………………..………… 5 Materials Provided…………………………….. 6 Additional Materials Required………………… 6 III. Overview and General Considerations………… 7 A. Preparation of Samples……………………… 7 B. Handling Glass Chips ………………………. 7 C. Incubation…………………………………… 8 IV. Protocol………………………………………… 8 A. Blocking and Incubation……………………. 8 B. Detection……………………………………. 11 Interpretation of Results……………………….. 12 VI. Troubleshooting Guide………………………… 18 VII. Reference List…………………………………. 19 II. V. Cytokine Antibody Arrays are RayBiotech patent-pending technology. RayBio® is the trademark of RayBiotech, Inc. RayBio® Mouse Cytokine Antibody Array G series 6 1