Download Feed-forward Control System for Fed

Abstract
Increasing clinical and industrial advances in the pharmaceutical world requires
technological improvements in cell culture development and monoclonal antibody (mAb)
production. Experiments performed with Chinese Hamster Ovary (CHO) cells producing mAbs,
have shown enhanced titers with a certain feed strategy. However implementing a feedback
control system is not preferable for large-scale manufacturing. In this paper, a method to create
feed-forward control with BioXpert, SCADA software is presented. The results are not
conclusive since there were no replicates to demonstrate reproducibility. Assuming that the
results are correct, it may be concluded that feed-forward control system was successfully
implemented on the fed-batch bioreactors via BioXpert software by using feed profiles generated
from previous feedback experiments. Titer results obtained from BioXpert feed-forward
experiments yielded higher results than manual feed-forward experiments and similar to previous
feedback experiments. For future work, it is recommended to repeat BioXpert feed-forward
experiment with two or more replicates maintaining the same cell line and using more frequent
feeding schedule by keeping total daily feeding amount the same.
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