Download AmpFlSTR Sinofiler PCR Amplification Kit User Guide (PN 4384256C)

Chapter 5 Experiments and Results
Artifacts
Artifacts and anomalies are seen in all molecular biological systems.
Artifacts are typically reproducible, and anomalies are
nonreproducible, intermittent occurrences that are not consistently
observed in a system, for example, spikes and baseline noise.
Artifacts have been seen in data produced on genetic analyzers when
using the Sinofiler kit. In amplified samples, artifacts in the noncalling region may appear in the blue (95–100 nt) dye. Low-level
artifacts in the calling region may appear in the blue (118 nt), green
(97, 120, and 189 nt), and black (95–100, 164 nt) dyes, depending on
the sensitivity of the instrument.
Figure 5-7 on page 5-27 shows examples of baseline noise and
artifacts in an electropherogram while using the Sinofiler kit.
Genotyping may result in the detection of these artifacts as off-ladder
alleles, or “OL Alleles.” You should consider possible noise and
artifacts when interpreting data.
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AmpFlSTR® Sinofiler™ PCR Amplification Kit User Guide