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18114045_AE.book Page 21 Friday, July 4, 2003 10:31 AM
2
Operation
Five sizes of sample loop are available:
Sample loop
Catalogue no.
Loop 10 µl, 25 MPa
18-1120-39
Loop 100 µl, 25 MPa
18-1113-98
Loop 500 µl, 10 MPa
18-1113-99
Loop 1 ml, 10 MPa
18-1114-01
Loop 2 ml, 10 MPa
18-1114-02
Two techniques can be used for filling the sample loop; partial or
complete filling.
Type of filling
Volume to load
Partial filling
Max. 50% of the sample loop volume
Complete filling
2–5 times the sample loop volume
2.3.2 Partial filling
Partial filling is used when high recovery is required. The sample
volume loaded should be, at maximum, 50% of the loop volume. The
volumetric accuracy and precision is that of the syringe. Partial filling
allows the injected volume to be changed without changing the loop
and does not waste sample. The sample loop must be completely filled
with buffer before the sample can be loaded.
Partial filling is achieved as follows:
1
Set the injection valve to position INJECT.
2
Disconnect the tubing from the column inlet and immerse it in a
Waste bottle.
3
Run the pump with buffer at low speed.
4
Load the syringe with the required volume of sample.
Note:
No more than half (50%) a loop volume of sample should
be loaded into the loop.
ÄKTAFPLC System Manual 18-1140-45 Edition AE
21