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Download Launchpad User Guide - Flow Cytometry Core Facility
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Chapter 42: Sequence Calculator First select a display within the MALDI-MS base window showing a spectrum of collected data, and choose Select peaks... from the "Processing" menu. The displayed window is shown in Figure 42.31. Figure 42.31 Select Peaks window Two modes of peak selection are available, peaks may be selected either automatically or manually. The automatic selection chooses the most intense peaks in a given mass range. For manual selection, masses of individual peaks may be typed, or indicated using the cursor. When selecting peaks, avoid peaks at low mass (e.g. below mass 300), as low mass fragments often occur at the same masses in digestions of peptides. In other words, the presence of such fragments is seldom of any use in identification of a particular peptide. Automatic peak selection Type the mass range in the Masses entry over which peak selection is to be performed. The number of peaks to select is specified by the Peaks option. Press List peaks to create the list of peaks from the chosen mass range. Cursors can be used to select a range of masses from a spectrum display. Position two cursors bracketing the mass range of interest and press the button. Masses will be set to the range marked by the cursors. Introduction 637 • • • • • •
