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Basics Bruker Daltonik GmbH the deviations from the central point of the remaining scores are ranked such that the smallest deviation has a rank of 1. Tied scores are assigned for a mean rank. The sums for the ranks of scores with positive and negative deviations from the central point are then calculated separately. A value S is defined as the smaller of these two rank sums. S is then compared to a table of all possible distributions of ranks to calculate p, the statistical probability of attaining S from a population of scores that is symmetrically distributed around the central point. As the number of used scores, n, increases, the distribution of all possible ranks S tends towards the z-distribution, so for an n of greater than 10 this distribution is used to calculate p. This test assumes that the compared sample sets originate at least from a common distribution. For details, please refer to F. Wilcoxon, "Individual Comparisons by Ranking Methods", Biometrics 1, pp 80-83 (1945). 6.4.1.4 Kruskal-Wallis Test In statistics, the Kruskal-Wallis one-way analysis of variance by ranks is a non-parametric method. Unlike the analogous one-way analysis of variance, the Kruskal-Wallis test does not assume a normal population. This, like many non-parametric tests, uses the ranks of the data rather than their raw values to calculate the statistic. Since this test does not make a distributional assumption, it is not as powerful as the ANOVA test. The hypotheses for the comparison of two independent groups are: • H0 (null hypothesis): The samples come from identical populations • Ha (alternative hypothesis): The samples come from different populations Notice that the hypothesis makes no assumptions about the distribution of the populations. These hypotheses are also sometimes written as testing the equality of the central tendency of the populations. The test statistic for the Kruskal-Wallis test is H. This value is compared to a table of critical values for U based on the sample size of each group. If H exceeds the critical value for H at some significance level (usually 0.05) it means that there is evidence to reject the null hypothesis in favor of the alternative hypothesis. For details, please refer to W. H. Kruskal and W. A. Wallis, "Use of ranks in onecriterion variance analysis", Journal of the American Statistical Association 47 (260): pp 583-621 (1952). 6.4.1.5 Anderson-Darling Test The Anderson-Darling test (AD test; Stephens, 1974) is used to test if a sample of data comes from a population with a specific distribution. It is a modification of the Kolmogorov-Smirnov (KS) test and gives more weight to the tails than does the KS test. The 6-28 ClinProTools User Manual, Version 2.2