1
Download Exercise 1
Transcript
Workshop on Bacterial Genomics 30 A results list will then be displayed as below (note that the whole list is not shown). We are now going to take the protein sequences of one of these genes and search for similar protein sequences in the genomes of P. falciparum, P. berghei and P. chabaudi using Omniblast. This uses the wublastp algorithm. The example below will take you through this process for one protein, but if you have time try one or two others. To start with use the sequence of a protein from T. annulata (TA02485) as this as been annotated as a hexose transporter homologue (HT1 homologue) which is the name of the gene in P. falciparum as it appears in the literature, and so it is likely that it is an orthologue of the protein in P. falciparum. 31 Click on the link for as shown below. This will take you through to the feature page for this gene. 32 To access the protein sequence to do an OmniBlast search click on the protein link. -140-
