Download ZOOM Studio Manual 1.1 - Bioinformatics Solutions Inc.
Transcript
Usage examples for ABI SOLiD data 1. ZOOM –i Yoruban.15M.csfasta –g all.lst -mm 2 ZOOM maps the color space reads in Yoruban.15M.csfasta to the human chromosomes listed in the file all.lst, allowing two errors (either two genome differences and no sequencing errors on color space read, or one genome difference and one color space sequencing error, or no genomic differences and two color space sequencing errors). 2. ZOOM –i A.15M.csfasta –g all.lst –mm 4 –de ZOOM maps the color space reads in A.15M.csfasta to the human chromosomes listed in the file all.lst, allowing four errors. 3. ZOOM –if Yoruban_F3.csfasta –ir Yoruban_R3.csfasta –pe –pemin 1495 – pemax 2027 –g all.lst –mm 2 –dcs ZOOM maps the color spaced paired-end reads in Yoruban_F3.csfasta and Yoruban_R3.csfasta to the human chromosomes listed in all.lst, allowing two errors. Two paired-end reads are mapped from 1495bp to 2027bp.