Download Ettan Digester Operating Instructions

Transcript
4 Operation
4.5 Editing a method
Dry
This is the step after washing. An optional Hold check box may be selected to pause the
instrument between the Wash and Dry step.
This step is used for drying the gel plugs before adding enzyme. This step may be done
in one of two ways:
•
Using a liquid with high content of organic solvent (e.g. 100% Acetonitrile) as final
wash protocol and then leaving the microplates in situ on Ettan Digester and allowing
them to air dry. Typical drying time is 60 to 90 minutes depending on local temperature and humidity conditions (1 mm gel thickness)
•
Perform drying in external drying equipment, for example a vacuum centrifuge.
Software
Description
command
Skip
Skips/includes the dry step.
Note:
The step is skipped if the box is selected.
Time
Selects the time to wait for the gel plugs to dry. It is possible to abort
drying (by pressing the Continue button) if manual inspection of the
microplates shows that the gel plugs have been dried sufficiently.
Ettan Digester Operating Instructions 28964985 AB
61