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Table of Contents General Instructions: SOP for BRB Behavioral Suite ………………………………………………… 3 Cleaning the Behavioral Suite ……………………………………………….. 4 General Experimental Design…………………………………………………. 6 Protocols for Behavioral Experiments Activity Monitor/ Open Field ………………………………………………… 7 Elevated Plus Maze (without tracking system) ………………………. 8 Elevated Plus Maze (with tracking system) ……………………………. 9 Pre‐pulse Inhibition ……………………………………………………………… 10 Y‐Maze ………………………………………………………………………………… 11 Social Interaction …………………………………………………………………. 15 Intruder/Resident ……………………………………………………………….. 17 Sociability & Preference for Social Novelty ………………………….. 18 Habituation/Dishabituation…………………………………………………. 20 Olfaction …………………………………………………………………………….. 21 Novel Object Recognition…………………………………………………….. 23 Morris Water Maze …………………………………………………………….. 24 Ultrasonic Vocalization Detector………………………………………….. 28 Forced Swim Test………………………………………………………………… 30 Fear Conditioning……………………………………………………………….. 31 Tail Suspension Test……………………………………………………………. 33 Rotarod………………………………………………………………………………. 34 T‐Maze……………………………………………………………………………….. 35 Passive‐ and Active‐Avoidance……………………………………………. 38 Contact Information Animal resources ……………………………………………………………….. 40 BRB Behavioral suite ………………………………………………………….. 40 2