Download Multiphor ll Electrophoresis System

Contents
Contents
1
Introduction
2
Safety information
3
Description of parts
4
Installation
5
Operation
5.1
5.2
5.3
5.4
5.5
5.6
5.7
5.8
6
Electrophoresis using ExcelGel SDS and buffer strips .............................. 20
Electrophoresis using buffer chambers ........................................................... 25
Isoelectric focusing using Ampholine PAGplate........................................... 28
Isoelectric focusing using CleanGel IEF ........................................................... 32
2-D electrophoresis using Immobiline DryStrip and ExcelGel SDS ..... 34
Electrophoretic transfer Introduction ............................................................... 35
Stock solutions ............................................................................................................. 42
Running conditions for precast gels .................................................................. 43
Maintenance
6.1
Recycling ........................................................................................................................ 47
7
Technical specifications
8
Trouble shooting
9
Multiphor II application kits and accessories
9.2
9.3
9.4
9.5
9.6
9.7
9.8
Buffer Strip Positioner ............................................................................................... 59
Immobiline DryStrip Reswelling Try ................................................................... 60
Immobiline DryStrip Kit ............................................................................................ 60
NovaBlot Kit ................................................................................................................... 61
FilmRemover ................................................................................................................. 62
Roller ................................................................................................................................. 63
Sample application accessories .......................................................................... 63
10 Ordering information
10.1
10.2
10.3
10.4
10.5
10.6
10.7
Multiphor II ..................................................................................................................... 67
MultiTemp III .................................................................................................................. 69
EPS Power Supplies ................................................................................................... 69
Precast gels and buffer strips ............................................................................... 70
Molecular weight and pI markers ....................................................................... 71
Carrier ampholytes .................................................................................................... 71
PlusOne electrophoresis chemicals ................................................................... 73
Multiphor ll Electrophoresis System User Manual 18-1103-43 Edition AK
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