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5.A. Detection of Amplified Fragments Using the Applied Biosystems® 3500 or 3500xL Genetic Analyzer with POP-4® Polymer (continued) 4. To create a new QC Protocol, navigate to the Library. Select “QC Protocols”, then select “Create”. Alternatively, a previously created QC Protocol may be used. 11272TA Assign a descriptive protocol name. Select the size standard created in Step 3. The settings for the QC protocol should be based on the internally validated conditions for the GenePrint® 10 System on the Applied Biosystems® 3500 or 3500xL Genetic Analyzer. Figure 5 shows one option for these settings. Figure 5. The Create New QC Protocol window. 16 Promega CorporaƟon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 TM392 · Revised 7/14 www.promega.com