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B
Appendix B Supplemental Procedures
Manually find the focus range
Figure 84 Examples of in-focus images.
9. Uncheck the Go Live check box in the Imager window to close the live-image
window.
Calculate, then set
the focal range
114
1. When the image is in focus, record the value of the Z distance in the Focus Status
pane. This is the nominal Z distance for this flowcell. Be sure to record the correct Z
distance value, not the value in the GoTo window (see Figure 85).
Applied Biosystems SOLiD® 4 System Instrument Operation Guide