Download Integrated Biology Workflow Guides

Contents
1 Before you begin 5
Introduction 6
Required items 8
Compliance 10
2 Working with Mass Profiler Professional 11
Where is MPP used in your experiment? 12
What is the metabolomics workflow? 13
Advanced operations covered in the MPP workflow guides 16
Using Mass Profiler Professional 17
3 Example experiments 19
Features of the example mass spectrometry experiments 20
Features of the example array experiment 22
Creating an expression analysis using the sample array experiment 23
4 Integrated Biology operations 35
Overview of operations 36
Results Interpretation 38
Pathway Analysis 61
NLP Networks 92
5 Reference information 109
Definitions 110
References 120
What’s new in Revision A
• This workflow guide is a complementary guide to the Agilent Metabolomics
Workflow - Discovery Workflow Guide (Agilent publication 5990-7067EN, Revision
B) The Metabolomics Workflow presents steps that precede the operations used
in the Integrated Biology Workflow.
• The Mass Profiler Professional wizard and workflow images are based on version
12.05.
• Formatting of text that appears in the left-hand margin helps guide you through
the operations.
• Operations are illustrated with flow charts that show you how the wizards are
navigated based on your experiment and selections.
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