Download Spatial intensity distribution analysis Matlab user guide

Load image
textbox
3. Set the correct parameters for the image file.
a.The GUI will attempt to obtain the information from the file itself. If it cannot, a dialog
box will appear and the correct image information can be manually set. The pixel size
and the beam waist radius in micron are needed. The pixel size depends on the
microscope settings (objective, zoom, size of image) and the beam size, which is
defined as the point spread function (PSF) mostly depend of the laser used and the
PSF for each laser line can be characterized using small fluorescent micropheres.