Download User Guide - Plexon Inc
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Unit Cleaning or more superimposed overlap templates did not reach the threshold. These overlap templates that failed to cross threshold are ignored for the rest of the analysis, and are shown as flat lines with unit shift values of -1,-1 in the Overlaps Analysis grid. 5 Inspect and Clean the Overlaps Some of the Overlap Templates are not “convincing”, just because of how the unit templates happened to superimpose to form the Overlap Template. The user should inspect the Overlaps that matched the Overlap Templates (the waveforms shown in yellow) to make sure they make sense as overlaps. To facilitate this, left-clicking-and-holding in one of the Overlap Templates grid cells that has yellow overlap candidate waveforms will select and highlight that candidate overlapping waveform and show it in the Waveforms and Clusters views. You should inspect the Overlap Templates grid for all unit permutations using the Next> and <Prev buttons. The candidate overlapping waveforms that are not acceptable (and should not be rescued) can be removed from consideration by right-clicking in the grid cell for the overlap and selecting Remove Matches for this Overlap Template from the context menu. If all matches for a particular unit permutation are not acceptable, they can all be removed by selecting Remove Matches for all Overlap Templates from the context menu. Conversely, it is useful to inspect whether a particular waveform shown in the Waveforms or Clusters views will be rescued or not. Clicking on a waveform in the Waveforms or Clusters views will display that waveform in all cells in the Overlaps Analysis grid. Also, it will find the closest Overlap Template (in any unit permutation) to the waveform, and highlight the cell for that Overlap Template in the Overlaps Analysis grid. The cell will be highlighted with a green box if the selected waveform matched the template to within Tolerance and therefore will be rescued, and the cell will be highlighted with a red box if the selected waveform did not match the Overlap Template to within Tolerance. (Note that the best Overlap Template is always found and shown, but even the best-matching Overlap Template might not have matched to within Tolerance.) In either case, the Tolerance that would be required in order to match the selected waveform to the Overlap Template is shown in white text in the upper left corner of the cell. This is useful to determine why a waveform that may look like an overlap in the Waveforms view did not match any of the Overlap Templates, and/or it can provide insight into what to set the Tolerance value to in order to detect and rescue what seems like overlaps. 6 Rescue the Overlaps Pressing the Rescue Overlaps button will rescue the overlap matching waveforms by creating two waveforms for each overlap. The rescued Version 4.0 197