Download User Guide - Plexon Inc

Transcript
Unit Cleaning
or more superimposed overlap templates did not reach the threshold. These
overlap templates that failed to cross threshold are ignored for the rest of the
analysis, and are shown as flat lines with unit shift values of -1,-1 in the
Overlaps Analysis grid.
5
Inspect and Clean the Overlaps
Some of the Overlap Templates are not “convincing”, just because of how the
unit templates happened to superimpose to form the Overlap Template. The
user should inspect the Overlaps that matched the Overlap Templates (the
waveforms shown in yellow) to make sure they make sense as overlaps. To
facilitate this, left-clicking-and-holding in one of the Overlap Templates grid
cells that has yellow overlap candidate waveforms will select and highlight
that candidate overlapping waveform and show it in the Waveforms and
Clusters views. You should inspect the Overlap Templates grid for all unit
permutations using the Next> and <Prev buttons.
The candidate overlapping waveforms that are not acceptable (and should not
be rescued) can be removed from consideration by right-clicking in the grid
cell for the overlap and selecting Remove Matches for this Overlap
Template from the context menu. If all matches for a particular unit
permutation are not acceptable, they can all be removed by selecting Remove
Matches for all Overlap Templates from the context menu.
Conversely, it is useful to inspect whether a particular waveform shown in the
Waveforms or Clusters views will be rescued or not. Clicking on a
waveform in the Waveforms or Clusters views will display that waveform in
all cells in the Overlaps Analysis grid. Also, it will find the closest Overlap
Template (in any unit permutation) to the waveform, and highlight the cell for
that Overlap Template in the Overlaps Analysis grid. The cell will be
highlighted with a green box if the selected waveform matched the template
to within Tolerance and therefore will be rescued, and the cell will be
highlighted with a red box if the selected waveform did not match the
Overlap Template to within Tolerance. (Note that the best Overlap Template
is always found and shown, but even the best-matching Overlap Template
might not have matched to within Tolerance.) In either case, the Tolerance
that would be required in order to match the selected waveform to the
Overlap Template is shown in white text in the upper left corner of the cell.
This is useful to determine why a waveform that may look like an overlap in
the Waveforms view did not match any of the Overlap Templates, and/or it
can provide insight into what to set the Tolerance value to in order to detect
and rescue what seems like overlaps.
6
Rescue the Overlaps
Pressing the Rescue Overlaps button will rescue the overlap matching
waveforms by creating two waveforms for each overlap. The rescued
Version 4.0
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