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Option Function Curve Fit Selects the curve fit algorithm used in the calibration. Linear - Performs a linear calibration. At least two standards are required. Linear to 0 - Performs a linear calibration forced through zero. Quadratic - Performs a quadratic fit on the data. At least three standards are required. Quad to 0 - Performs a quadratic fit with the data forced through zero. At least two standards are required. Measure Stds Toggles between internal calibration (Yes) and external calibration (No). Choose Yes to measure the standards and calibrate using the concentrations entered on the Standards screen. When ready, press Calibrate. At the prompt, insert the first standard in the beam and press Run. Repeat for the remaining standards in order. Choose No to specify absorbance values for the standards from an external calibration. When ready, press Calibrate. Enter the absorbance value of the first standard at the prompt and press Enter. Repeat for the remaining standards in order. Timer(s) (for AquaMate models only) Allows you to add up to 4 timers in a method for specific purposes. For each timer, define the following: Title – Select a name that indicates the purpose of the timer (Timer, Wait, Shake, Invert, Swirl, Boil or Heat). Duration – Specify a delay time from 1 to 100 seconds in digital format with a period separator (e.g., 00.01 to 99.59). Action – Select whether to display a user prompt when the delay time has passed. Choose Pause to display a user prompt with three choices (Stop, Zero or Continue). Choose Continue to skip the user prompt. After the delay time has passed, the system automatically proceeds to the next task in the measurement sequence. Note: Timers can be used with a sipper accessory that is in Auto mode. You cannot use a timer with a cell programmer in Auto mode. See Timer Function Keys for more information. 32 Spectrophotometer User Guide