Download RNA Assay User Guide

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Contents
Specifications ......................................................................................................... 3
Assay Specifications .......................................................................................... 3
Sample Conditions ............................................................................................. 3
Kit Contents ....................................................................................................... 4
Safety Warnings and Precautions.......................................................................... 5
Preparation Procedures ......................................................................................... 6
Additional Items Required .................................................................................. 6
Preparing the Gel-Dye Solution .......................................................................... 6
Preparing the RNA Samples and RNA Ladder .................................................... 7
Preparing the Buffer Tube .................................................................................. 8
Preparing the Chip ............................................................................................. 8
Inserting a Chip into the LabChip GX Touch/GXII Touch Instrument ................. 10
Running the Assay ........................................................................................... 12
Refreshing the Gel-Dye Solution ...................................................................... 15
Storing the Chip ............................................................................................... 15
Chip Cartridge Cleaning ................................................................................... 16
Results .................................................................................................................. 18
RNA Ladder Result .......................................................................................... 18
RNA Eukaryote Sample Result ......................................................................... 18
RNA Prokaryote Sample Result ........................................................................ 19
RNA mRNA Sample Result .............................................................................. 19
Troubleshooting ................................................................................................... 20
LabChip Kit Essential Practices........................................................................... 26
General ............................................................................................................ 26
Reagents .......................................................................................................... 27
Chips ................................................................................................................ 28
Samples ........................................................................................................... 31
Chip Well Aspiration Using a Vacuum ................................................................. 32
Reordering Information ........................................................................................ 33
Customer Technical Support ............................................................................... 34
Licenses and Rights of Use ................................................................................. 35
PN CLS140164, Rev. C
RNA Assay User Guide