Download P81 Single Sided Vertical Electrophoresis System User Manual

Single Sided Vertical Electrophoresis System
P81
Operating and Maitnenance Manual 7007341 Rev. 0
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Preface
MANUAL NUMBER 7007341
0
--
5/3/12
Transfer to Marietta (was The Puffin 11/2002)
REV
ECR/ECN
DATE
DESCRIPTION
Thermo Scientific
By
Vertical Electrophoresis System
i
Preface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Warning To avoid the risk of personal shock, always disconnect the gel box from the power supply. Further, the
power supply must be equipped with a shut-down-on-disconnect circuit. Running conditions for this unit
should not exceed the name plate readings found on the lower buffer chamber. Do not move the unit unless the
power source to the unit has been disconnected. s
Statement of Proper Use: Use this product only for its intended purpose as described in this manual. Do not use
this product if the power leads are damaged or if any of its surfaces are cracked.
This Owl System is designed to meet IEC 1010-1 safety standards (IEC 1010-1 is an internationally accepted
electrical safety standard for laboratory instruments).
Material in this manual is for information purposes only. The contents and the product it describes are subject to
change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
©2012 Thermo Fisher Scientific. All rights reserved.
ii
Vertical Electrophoresis System
Thermo Scientific
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo products will be available at www.thermofisher.com.
4
Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4 Always dissipate extreme cold or heat and wear protective clothing.
4 Always follow good hygiene practices.
4 Each individual is responsible for his or her own safety.
Thermo Scientific
Vertical Electrophoresis System
iii
Preface
Do You Need Information or Assistance on
Thermo Scientific Products?
If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:
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Our Sales Support staff can provide information on pricing and give you quotations. We can
take your order and provide delivery information on major equipment items or make
arrangements to have your local sales representative contact you. Our products are listed on the
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Our Service Support staff can supply technical information about proper setup, operation or
troubleshooting of your equipment. We can fill your needs for spare or replacement parts or
provide you with on-site service. We can also provide you with a quotation on our Extended
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Whatever Thermo Scientific products you need or use, we will be happy to discuss your
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When more extensive service is necessary, we will assist you with direct factory trained
technicians or a qualified service organization for on-the-spot repair. If your service need is
covered by the warranty, we will arrange for the unit to be repaired at our expense and to your
satisfaction.
Regardless of your needs, our professional telephone technicians are available to assist you
Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by
telephone or fax. If you wish to write, our mailing address is:
Thermo Fisher Scientific
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International customers, please contact your local Thermo Scientific distributor.
iv
Vertical Electrophoresis System
Thermo Scientific
Table of Contents
Thermo Scientific
Section 1
General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Unpack and Check Your Order . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Section 2
Using the System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Running the Gel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
After the Gel Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Running Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2
Section 3
Technical Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Glass . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Spacers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Acrylamide Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Reagent Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Offset vs. Notched Glass . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Notched Alumina Plates - for P8DS Only . . . . . . . . . . . . . . . . . . . . .3-3
Combs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-3
Section 4
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Section 5
Care and Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Section 6
Optional Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Vertical Electrophoresis System
v
Section 1
General Information
The Single Sided Vertical Electrophoresis System is simple, rugged and
provides excellent results. The upper buffer chamber (UBC) of the single
sided vertical system extends the length of the gel to distribute heat evenly
across the entire gel surface. Side clamps press glass plates against asilicome
gasket to provide univlorm pressure and form a leak proof seal. This heat
and pressure distribution system includes a notched alumina ceramic plate
which may be used in front of the notched glass for cooler, faster runs.
Unpack and Check
Your Order
Before starting, unpack the unit and inventory your order. If any parts are
missing, contact Technical Services immediately.
Reference the order or catalog number on your invoice and check the
corresponding parts list.
Table 1-1. Parts List
Description
Part Number
Lid with attached Power Supply Leads
-
Upper Buffer Chamber
-
Lower Buffer Chamber
-
Blank glass plates 3/32" Thick
P7-10G
Size
Notched glass plates 3/32" Thick
Size
Notched Alumina Plates 1.0mm Thick
Size
Thermo Scientific
10cmW x 10cmL
P7-10R
10cmW x 10cmL
P7-10RA
10cmW x 10cmL
Spacers, 0.8mm Thick
P7-SC
Well Comb, 10 teeth, 0.8mm thick
MP-10C
Vertical Electrophoresis System
1-1
Section 1
General Information
Table 1-2. Specifications
Unit/Model Number
P81
Gel size
10cmW x 10cmL & 10cmW x 8cmL
Upper Buffer Chamber Capacity
100ml
Lower Buffer Chamber Capacity
50ml
Total Running Buffer
150ml
Total Buffer Capacity
150ml
Current, Constant
15-35mA/gel
Time Requirements
30-90 min.
Sample Capacity
10
Dimensions (cm) H x W x D
12.7 x 18.4 x 8.3
Glass Size (cm)W x L
10 x 10
Power Supply Leads
Comb (2)
Notched Alumina
Plate
Upper Buffer
Chamber (UBC)
Notched Glass
Super Safe
Lid
Blank Glass
Spacer Set
Lower Buffer
Chamber (LBC)
Figure 1-1. Exploded Parts Diagram
1-2
Vertical Electrophoresis System
Thermo Scientific
Section 2
Running the Gel
Using the System
Note Owl offers a multiple gradient caster and a gel caster. See Section 6
for details.
1. After the gel is cast, place the gel with the notched or offset glass plate
facing the inside of the upper buffer chamber. The gel cassette must be
placed squarely on the corners in order to provide a good seal with the
gasket and avoid leakage of buffer from the upper buffer chamber to
the lower buffer chamber.
2. Add running buffer to the upper buffer chamber making sure the
running buffer is 3mm below the top of the blank glass, ensuring
sufficient contact with the top of the gel surface. Be sure that the
running buffer is not leaking from the upper buffer chamber to the
lower buffer chamber. If buffer is leaking, you will need to drain the
UBC and reset the cassettes.
3. Remove combs by gently pulling straight up from the gel. Carefully
load samples into the wells formed by the comb. Rinse wells with
water.
4. Add buffer to the lower buffer chamber to approximately 2-3mm
above the base of the gel using the fill line as a guide (max. fill). The
bottom end of the gel/agarose assembly should be in contact with the
running buffer.
5. Set the safety lid onto the unit so that the power supply leads are
connected. Begin the gel run.
Table 2-1. Maximum Buffer Volumes
Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P81
Upper Buffer Chamber . . . . . . . . . . . . . . . . . .100ml
Lower Buffer Chamber . . . . . . . . . . . . . . . . . . .50ml
Thermo Scientific
Vertical Electrophoresis System
2-1
Section 2
Using the System
Running Conditions
Running conditions depend on several parameters:
•
Buffer system used
•
Whether or not heating would affect subsequent processing of the
proteins or gel
•
Thickness of the gel
•
How fast the gel will be run – for example, set it up in the late
afternoon and have the gel done the next morning, or have it done in
45 minutes or less.
A guideline for 2nd dimension gels - the range would be 30-80mA
constant current.
Example: For an SDS-PAGE gel in the P8DS that is 0.8mm thick and
temperatures over 37°C are not an issue, 40mA per gel is appropriate. If
the gel were 1.5mm thick, the setting could be 60mA or higher.
Table 2-2. Recommended Running Conditions
Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P81
Constant Current . . . . . . . . . . . . . . . . .15-35mA/gel
Time Requirements . . . . . . . . . . . . . . .30-90 minutes
After the Gel Run
1. Turn off power supply.
2. Remove the lid. Slide and lift the upper buffer chamber from the lower
buffer chamber and drain buffer chambers separately.
3. Loosen wing knobs and slide side clamps to remove gel cassettes. The
gel is ready for staining and blotting.
Contact Technical Services or see the product catalog for additional
staining and blotting accessories.
2-2
Vertical Electrophoresis System
Thermo Scientific
Section 3
Glass
Technical Tips
BLANK
The plate which faces you during electrophoresis. All
gel sandwiches require one piece of blank glass.
BLANK
NOTCHED
The plate which faces the chamber during electrophoresis. Spacers are placed over the "ears" of the
plate when casting vertical gels. Buffer accesses the gel
between the ears.
NOTCHED
OFFSET
Offset plates may be used in place of notched plates.
They require sponge tips mounted on the spacers.
Sponge tips take the place of the "ears", and prevent
buffer from running out of the upper buffer chamber
from the sides.
OFFSET
FROSTED
Frosted plates are used for vertical agarose electrophoresis. One side of
the plate has a rough surface to prevent agarose from sliding down.
Spacers
Thermo Scientific
STANDARD
Protein spacer sets include two side spacers and
one bottom spacer. Spacers and combs must be
of identical thickness to be used together.
SPONGE TIP
SPACER
Vertical Electrophoresis System
3-1
Section 3
Technical Tips
Reagent Information
RUNNING BUFFER
TGS
Tris - 3.0285g/L
Glycine - 14.4g/L
SDS - 1.0g/L
pH 8.3 (Laemmli, 1970)
q.s. to 1L
Note For Native Protein Electrophoresis, do not add SDS. s
Table 3-1. Sample Buffer
2X Concentration
Stock
/L
/10 mL
Final Concentration
With Sample*
2%
SDS
20g
0.2
1%
10%
BME
10mL
0.1
5%
250mM
Tris
6.057g
.0606g
125mM
30%
Glycerol
300 mL
3 mL
15%
0.002%
Bromo Phenol Blue
.02g
.0002g
0.001%
* add sample buffer 1:1 with sample solution.
Caution 2X Sample Buffer containing 2-mercaptoethanol should be
prepared in a fume hood. 0.2M (final concentration) Dithiothreitol (DTT)
may be used in place of 2-mercaptoethanol. DDT should be added before
use and made fresh. s
Acrylamide Solution
Stock acrylamide solution for Table 3-2: = 29.2g Acrylamide and .8g bisAcrylamide, q.s. 100mL H2O
Table 3-2. Gel Preparation (SDS-Page continuous buffer system)
% Acrylamide*
Stock Solution
20.0
15.0
12.5
10.0
5.0
Acrylamide-Bisacrylamide (30:0.8)
20.0
15.0
12.5
10.0
5.0
0.5 M Sodium Phosphate Buffer pH 7.2
6.0
6.0
6.0
6.0
6.0
10% (w/v) SDS
0.3
0.3
0.3
0.3
0.3
Water
2.2
7.2
9.7
12.2
17.2
1.5% (w/v) APS
1.5
1.5
1.5
1.5
1.5
TEMED
0.015
0.015
0.015
0.015
0.015
* The columns represent volumes (ml) of stock solutions required to prepare
30ml of gel mixture.
3-2
Vertical Electrophoresis System
Thermo Scientific
Section 3
Technical Tips
Combs
STANDARD
• 0.5mm(A), 0.8mm (C) and 1.5mm (D)
thicknesses
PREPARATIVE
• One long well and one marker lane
Standard
Preparative
CUSTOM COMBS
Call Technical Services for more information.
Notched Alumina
Plates - for P8DS Only
Offset vs. Notched
Glass
Thermo Scientific
Notched Alumina plates can take the place of the notched glass when
casting and allows for better heat exchange than glass. This is important
when the protein is heat sensitive or if a gel needs to be run a little faster
without the negative effects of heating. Heating the gel during the run can
cause smiling and other distortions of the gel.
All units require a blank piece of glass and an
offset or notched piece of glass. Offset glass is glass
that is about 2cm shorter than the blank piece
without "ears" on the sides. Notched glass has two
"ears" that are left behind when a cut is made in
the middle of the top of the glass. Both offset glass
and notched glass allow the gel and samples to
make contact with the upper buffer chamber.
Offset glass has to be used with sponge tips, which
take the place of the notches on the glass. The
advantage of offset glass is that this glass is more
rigid. Notched glass is easier to use and does not
require the addition of sponge tips.
SPONGE TIP
SPACER
Vertical Electrophoresis System
3-3
Section 4
Troubleshooting
Problem
Cause
Solution
Broad lanes at bottom of gel
Will occur when adjoining lanes are loaded
Normal in gradient gels
with dissimilar samples.
Gel has not polymerized properly at wells.
Degas gel solution before casting and increase
APS and TEMED concentrations. The comb can
be wiped with TEMED just prior to casting to
improve polymerization.
Salt concentration is too high in sample.
Dialyze sample or use desalting column.
The upper buffer chamber is leaking either
through the gel or along the sides.
Check gel to make sure that it is a solid slab
inside the glass and check the setup of the apparatus to ensure a secure seal with gasket.
Overloading of sample.
Use less protein or sample when loading.
Sample has precipitated.
Centrifuge sample before adding sample buffer
or use a lower % acrylamide gel.
Frowning of outside lanes
Leakage of buffer along sides or along
spacers inside the gel assembly.
Do not move spacers after polymerization and
make sure that gasket is seated firmly against
the glass. Always load your samples with an
empty lower buffer chamber so that leaks are
caught before you begin the run.
Double bands (“doublets”)
If using a reducing agent, prepare fresh sample
Due to reoxidation or insufficient reduction
buffer every 30 days. Increase the concentration
of the sample.
of 2-mercaptoethanol or dithiothreitol in sample.
Skewed bands
Streaked bands
Glass cracks when putting gel
Gel is too thin for the clamping system.
assembly in unit
Use glass appropriate for the unit. If this is not
possible, use an extra piece of blank glass to
take up the space. If the clamps are used with
their flat side against the glass, thinner glass
may often be used.
Wash gasket after each use to remove salts. If
gasket is old and has lost its flexibility, it may
Gasket is old or flattened making it impossi- need to be replaced. If unit has been previously
ble to make a good seal.
overtightened, the gasket may need to be
removed and reseated. A cracked and dry gel
often is an indicator of overheating.
Thermo Scientific
Vertical Electrophoresis System
4-1
Section 4
Troubleshooting
Problem (continued)
Cause (continued)
Solution (continued)
Buffer is too diluted
Check buffer recipe; remake buffer and
try again. See if voltage produced by the
current you are running at is the same. If
it differs significantly, your buffer may not
have been made up correctly.
Upper buffer chamber is leaking
Make sure that the gel assembly is seated
firmly against the gasket. Remove gasket,
wash in warm water to remove excess
salts, and place the gasket back in the
groove. If the clamps have been overtightened
in the past, the gasket can be pushed
too far into the gasket groove and will not
make a seal.
Running at too low a current
Use running conditions as stated in table
3-2. When running at constant current, the
current value is per gel.
Buffers are too concentrated
Check buffer recipe; remake and try again.
If voltage is lower than usual when running
at constant current, the buffer is probably
too dilute.
Voltage or current set too high
Turn down current setting
Smiling of dye front
Center of gel is running hotter than ends
Turn down current setting
Bands spreading outward
Diffusion of sample when loading
Make sure that the samples are loaded
quickly and the power is applied as soon
as possible after loading.
Diffusion of sample during run in stacking
gel
Increase % of stacking gel or increase current
by 25% when stacking.
Lower ionic strength of sample
Match the ionic strength of the sample with
that of the gel.
Longer run time
Running too fast
Bands are narrower than sample wells
4-2
Vertical Electrophoresis System
Ionic strength of sample is higher than that Desalt the sample or use sample buffer of
of the gel
the same strength as the gel.
Thermo Scientific
Section 5
Care and Cleaning
A few tips about caring for your system follow.
Caution Organic solvents cause acrylic to “craze” or crack. Clean all acrylic
systems with warm water and a mild detergent. Do not use ethanol or
other organic solvents to clean these products. Do not autoclave, bake, or
microwave your unit. Temperatures over 50°C can damage the acrylic. s
Note If an RNase free electrophoresis system is desired, there are various
methods to rid the system of RNA contamination. For fast and easy
decontamination, use RNase Away®*. Spray, wipe or soak labware with
RNase Away then wipe or rinse the surface clean; it instantly eliminates
RNase. RNase Away eliminates the old methods that include treatment
with 0.1% Diethyl Pyrocarbonate (DEPC) treated water and soaking in
diluted bleach. DEPC is suspected to be a carcinogen and should be
handled with care. This electrophoresis system should never be autoclaved,
baked, or placed in a microwave. s
To order RNase AWAY®, contact Technical Services:
Part Number . . . . . . . . . . . . . . . . . . . . . .Description
7000 . . . . . . . . . . . . . . . . . . . . . . . . . . .250ml bottle
7002 . . . . . . . . . . . . . . . . . . . . . .475ml spray bottle
7003 . . . . . . . . . . . . . . . . . . . . . . . . . . .1 liter bottle
7005 . . . . . . . . . . . . . . . . . . . . . . . . . . .4 liter bottle
*Rnase AWAY® is a registered trademark of Molecular BioProducts
Thermo Scientific
Vertical Electrophoresis System
5-1
Section 5
Care and Cleaning
Care of Acrylic
The following chemical compatibility chart is supplied for the convenience
of our customers. Although acrylic is compatible with most solvents and
solutions found in the biochemical laboratory, some solvents can cause
substantial damage. Keep this chart handy to avoid harm to your apparatus
by the use of an inappropriate solvent.
Codes:
S - Safe (no effect, except possibly some staining)
A - Attacked (slight attack by, or absorption of, the liquid)
(slight crazing or swelling, but acrylic has retained most of its strength)
U - Unsatisfactory (softened, swollen, slowly dissolved)
D - Dissolved (in seven days, or less)
5-2
Vertical Electrophoresis System
Thermo Scientific
Section 5
Care and Cleaning
Table 6-1. Chemical Compatibility for Acrylic-Based Products
Chemical
Code Chemical
Code Chemical
Code
Acetic acid (5%)
S
Ethyl alcohol (50%)
A
Naptha
S
Acetic acid (Glacial)
D
Ethyl alcohol (95%)
U
Nitric acid (10%)
S
Acetic Anhydride
A
Ethylene dichloride
D
Nitric acid (40%)
A
Acetone
D
Ethylene glycol
S
Nitric acid concentrate
U
Ammonia
S
2-Ethylhexyl Sebacate
S
Oleic acid
S
Ammonium Chloride (saturated)
S
Formaldehyde (40%)
S
Olive oil
S
Ammonium Hydroxide (10%)
S
Gasoline, regular, leaded
S
Phenol 5% solution
U
Hydroxide (10%)
S
Glycerine Heptane (commercial grade)
S
Soap solution (Ivory)
S
Ammonium Hydroxide concentrate
S
Hexane
S
Sodium carbonate (2%)
S
Aniline
D
Hydrochloric acid (10%)
S
Sodium carbonate (20%)
S
Benzene
D
Hydrochloric acid concentrate
S
Sodium chloride (10%)
S
Butyl Acetate
D
Hydrouoric acid (40%)
U
Sodium hydroxide (1%)
S
Calcium chloride (saturated)
S
Hydrogen peroxide (3% solution)
S
Sodium hydroxide (10%)
S
Carbon tetrachloride
U
Hydrogen peroxide (28% solution)
U
Sodium hydroxide (60%)
S
Chloroform
D
Isooctane
S
Sodium hydrochlorite (5%)
S
Chromic acid (40%)
U
Isopropyl alcohol (100%)
A
Sulfuric acid (3%)
S
Citric acid (10%)
S
Kerosene (no. 2 fuel oil)
S
Sulfuric acid (30%)
S
Cottonseed oil (edible)
S
Lacquer thinner
D
Sulfuric acid concentrate
U
Detergent Solution (Heavy Duty)
S
Methyl alcohol (50%)
A
Toluene
D
Diesel oil
S
Methyl alcohol (100%)
U
Trichloroethylene
D
Diethyl ether
U
Methyl Ethyl Ketone
U
Turpentine
S
Dimethyl formamide
U
Methylene chloride
D
Water (distilled)
S
Dioctyl phthalate
A
Mineral oil (white)
S
Xylene
D
Ethyl acetate
D
This list does not include all possible chemical incompatibilities and safe compounds. Acrylic products
should be cleaned with warm water, a mild detergent such as Alconox™, and can also be exposed to a
mild bleach solution (10:1). In addition, RNAse removal products are also safe for acrylic. Contact
Technical Services with any questions.
Thermo Scientific
Vertical Electrophoresis System
5-3
Section 6
Optional Equipment
Contact the Technical Services department to order replacement parts.
Table 6-1. Replacement Parts
Thermo Scientific
Description
P81
Power Supply Leads
PSL-5
Clamp Assemblies
P8-CL
Replacement Gaskets
R12009
Blank Glass Plates 3/32” thick
P7-10G, 10cmW x 10cmL
Notched glass plates 3/32" Thick
P7-10R, 10cmW x 10cmL
Frosted Notched glass plates 3/32" Thick
P7-10FR, 10cmW x 10cmL
Frosted Blank Glass Plates 3/32" Thick
P7-10FG, 10cmW x 10cmL
Notched Alumina Plates 1.0mm Thick
P7-10RA, 10cmW x 10cmL
Spacers, 0.5mm Thick
P7-SA
Spacers, 0.8mm Thick
P7-SC
Spacers, 1.5mm Thick
P7-SD
Blocking Plate for Single Gel Operation
P8DS-016
Spacer Placer (pkg of 3)
JG4-PL
Vertical Electrophoresis System
6-1
Section 6
Optional Equipment
Table 6-2. P81 Comb Options
6-2
Vertical Electrophoresis System
Catalog
Number
Comb
Type
Number of Thickness of
Teeth
Teeth (mm)
Width of
Teeth (mm)
Estimated Well
Volume (ul)
MP-6A
Well
6
0.5
11.1
89
MP-6C
Well
6
0.8
11.1
142
MP6D
Well
6
1.5
11.1
266
MP8A
Well
8
0.5
7.7
62
MP-8C
Well
8
0.8
7.7
99
MP-8D
Well
8
1.5
7.7
185
MP-10A
Well
10
0.5
5.7
46
MP-10C
Well
10
0.8
5.7
73
MP-10D
Well
10
1.5
5.6
134
MP-12A
Well
12
0.5
4.3
34
MP-12C
Well
12
0.8
4.3
55
MP-12D
Well
12
1.5
4.3
103
MP-15A
Well
15
0.5
2.9
23
MP-20A
Well
20
0.5
1.6
13
XCM
Custom
0.5, 0.8, 1.5, 2.0,
3.0
Thermo Scientific
Section 6
Optional Equipment
MULTIPLE GRADIENT CASTER
The Multiple Gradient Caster System
features an easy-to-use casting base
and specially designed spacer plates
for quick casting of high quality
linear or gradient gels. Using the
acrylic and foam spacer plates allows
you to cast from one to five gels
simultaneously. A silicone gasket
provides a leak proof seal and the
casting port allows the casting of
gradient gels from the base of the
caster.
Rabbit . . . . . . . . . . . . . . . . . . . . . . . . . . . . .P7-CST
Gel Size . . . . . . . . . . . . . . . . . . . . .10cmWX 10cmL
GEL CASTER
The patented Gel Casting System provides a simple
method of casting acrylamide gels without taping or
special sealing of the gel plates. Plastic pouches hold
glass plates and spacers snugly together in the casting
stand while pouring. These pouches meet specific
measurement tolerances to allow for a tight fit
around glass plates. Gels may be cast ahead of time
and sealed inside the plastic pouch, creating your
own pre-cast gels. Up to four gels may be cast at one
time.
Joey . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .JGC-4
Gel Size . . . . . . . . . . . . . . . . . . . . .10cmWX 10cmL
RELATED PRODUCTS
•
•
•
•
•
•
Thermo Scientific
Panther Semi-dry Electroblotter
Puffin Single Sided Vertical Gel Electrophoresis System
Bandit Tank Style Electroblotter
Powdered Buffers
Silver Stain
Pro Blue
Vertical Electrophoresis System
6-3
Thermo Scientific
Rev. 0 9/12
REGISTERED
ISO
9001
If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada) or
1-740-373-4763. We’re ready to answer your questions on equipment warranty, operation, maintenance, service, and special applications. Outside the USA, contract your local distributor for warranty information.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL, OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages to lost profits or
loss of products.
Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give
prior approval for return of any component or equipment. At Thermo’s option, all non-conforming parts must be returned to
Thermo postage paid and replacement parts are shipped FOB destination.
During the first thirty-six (36) months, component parts proven to be non-conforming in material or workmanship will be
replaced at Thermo’s expense, including labor. Installation, calibration and certification is not covered by this warranty
agreement. The Technical Services Department must be contacted for warranty determination and direction prior to performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.
The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows shipping time
so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection
extends to any subsequent owner.
THERMO FISHER SCIENTIFIC OWL PRODUCTS WARRANTY USA
Section 7
Warranty Information
Vertical Electrophoresis System
7-1
7-2
Vertical Electrophoresis System
Rev. 0 9/12
REGISTERED
ISO
9001
If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA or Canada) or
1-740-373-4763. We’re ready to answer your questions on equipment warranty, operation, maintenance, service, and
special applications. Outside the USA, contract your local distributor for warranty information.
Your local Thermo Sales Office is ready to help with comprehensive site preparation information before your equipment
arrives. Printed instruction manuals carefully detail equipment installation, operation and preventive maintenance.
THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL, OR
IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY.
Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages to lost profits
or loss of products.
Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the
equipment or to the component part beyond the original warranty period. The Technical Services Department must give
prior approval for return of any component or equipment. At Thermo’s option, all non-conforming parts must be returned
to Thermo postage paid and replacement parts are shipped FOB destination.
During the first thirty six (36) months, component parts proven to be non-conforming in material or workmanship will be
replaced at Thermo’s expense, excepting labor. Installation, calibration and certification is not covered by this warranty
agreement. The Technical Services Department must be contacted for warranty determination and direction prior to performance of any repairs. Expendable items, glass, filters and gaskets are excluded from this warranty.
The Warranty Period starts two months from the date your equipment is shipped from our facility. This allows shipping
time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner.
THERMO FISHER SCIENTIFIC OWL PRODUCTS WARRANTY INTERNATIONAL
Section 7
Warranty Information
Thermo Scientific
Thermo Fisher Scientific
401 Millcreek Road
Marietta, Ohio 45750
United States
www.thermofisher.com