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8.1.1
If there is only one standard vial in the kit ........................................................................ 12
8.1.2
If there are multiple standard vials in the kit .................................................................... 12
8.2
9
Serial dilution preparation ........................................................................................................ 13
Performing the assay ........................................................................................................................ 14
10
Setting Up Flow cytometers .......................................................................................................... 15
10.1
Fluorescence channels .............................................................................................................. 15
10.2
Preparing instrument setup beads ............................................................................................ 15
10.3
Setting up a display layout/template ........................................................................................ 16
10.4
Running the setup beads........................................................................................................... 16
11
Analyzing the samples ................................................................................................................... 19
12
Technical hints .............................................................................................................................. 19
13
Troubleshooting ............................................................................................................................ 21
14
Blank Plate Layout ......................................................................................................................... 22
4
Rev 1.3.4