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6 Troubleshooting
6 Troubleshooting
6.1 UV curve problems
Error symptom
Possible cause
Corrective action
Ghost peak
Dirt or residues in the
flow path from previous runs. Air in the
eluents.
Clean the system. Make sure air is
removed.
Residue in the column
from previous runs
Clean the column according to the
column instructions.
Incorrect mixer function
Check the mixer function by placing a stirrer bar on top of the mixer
housing. The stirrer bar should rotate when the system is in Run
mode. The mixer function can also
be checked by running the installation test.
Dirty UV cell
Clean the UV cell by flushing Decone 90, Deconex 11 or equivalent.
Impure buffer
Check if the signal is still noisy with
water.
Air in the pump or in
the UV cell
Purge the pump according to
Pump User Manual. Run a system
wash with buffer.
Noisy UV-signal, signal drift or instability
ÄKTAprime plus Operating Instructions 28-9597-89 AA
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