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UVS SOFTWARE for UVIKON 900 / XS /XL
USER MANUAL
Bezier
A Bezier curve is an approximation to a curve which would pass through all the control
points. The method used here for defining such a curve approximation is called the
Bezier-Bernstein approximation.
2.5.1.4.
Calculation
The calculation function allows definition of mathematical functions which can be used to modify the
curve data. “S” is used to denote the sample itself and calculations are defined simply using “S” and
the appropriate factor (F) and signs:
Addition:
Enter
F+S
Subtraction:
Enter
S-F
Multiplication:
Enter
F*S
Division:
Enter
S/F
For example it is also possible to subtract an offset from each point in the curve and then double the
signal level and this would be programmed as follows:
Click on the Calculation checkbox and then enter the required formula, in this example the formula
would simply be 2*(S-0.2).
Caution
Please note that spaces are not permitted in the formula.
2.5.1.5.
Peak Detection
This calculation mode can be used with the system methods: Wavelength Scan, Time Drive and Auto
Rate Assay.
Peak detection can be linked to a method and carried out during or after measurement. To activate
peak detection select “Peak detection” in the calculation parameter editor window.
A peak is detected when the slope of the curve changes from positive to negative, a valley is detected
when the slope changes from negative to positive.
After selecting “Peak detection” it is possible to set the following parameters:
Min. Abs
The Min. Abs. parameter defines the absolute minimum value for a
peak or valley to be detected, any peak or value with an absorbance
value lower than the defined value will be ignored.
Threshold
The threshold is the difference in height on the Y-axis between two
peaks or valleys. If the difference between valley and peak is lower
than the set threshold then the peaks/valleys will not be detected.
Peak / Valley
The selection defines whether the calculation is to be made for peaks
only, valleys only or both peaks and valleys.
UVS Software for
UVIKON 900/XL/XS
Manual Version 2.04, Oct. 2004
Page 95 of 124