Download Imagene User Guide

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Segmented - Displays an image of the segmentation, or pixel determination, that
will be performed during quantification. The red pixels represent signal values
and the green pixels represent background values. Black means the pixel is
ignored.
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Composite - Displays an overlay between the Original and Segmented images.
This image is key to determining if the best settings have been entered on the
Measurements tab. Use this view to modify the settings until the desired signal
and background values are included while removing contaminants.
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Histogram - Represents the distribution of pixels along the intensity scale for the
spot. The y-axis (vertical) represents the number of pixels and the x-axis
(horizontal) represents the range of intensities of pixels across the spot. The
histogram is intended for a simple qualitative preview and should not be used for
any form of data analysis. Within the histogram, colored vertical bars indicate
specific values. The colors and their meaning are:
o Blue – Signal Mean Value
o Yellow – Signal Median Value
o Cyan – Signal Mode Value