Download - Bio-Rad

Contents
ProteOn Sensor Chips
1
Storing Sensor Chips Opening a Sensor Chip Initializing a Sensor Chip Setting Up a Protocol 2
3
3
3
Running Experiments with Sensor Chips
4
Conditioning Chips Ligand Immobilization Desired Ligand Immobilization Level
Optimizing Immobilization Conditions
GLX Sensor Chips
Activation
Ligand Immobilization
Deactivation NLC Sensor Chip Ligand Capture HTX Sensor Chips Activation and Ligand Capture LCP Sensor Chip and Liposome Capturing Kit Activation, Conditioning, and Liposome Capture
GLC Lipid Kit Surface Modification
Partial Surface Modification
Liposome Capture
Troubleshooting Ligand Immobilization
Amine Coupling with GLX Chips
Typical Ligand Buffer Conditions
Activation Quality
Ligand Activity
Ligand Capture with the NLC Chip
Stabilization Analyte Injection Troubleshooting Analyte Injection
Nonspecific Binding
Electrostatic NSB
Non-Electrostatic NSB
Techniques for Reducing or Eliminating NSB on All Sensor Chips
Bulk Effect
Correcting for Bulk Effect
Bubble Formation
Tips for Reducing Bubble Formation
Regeneration GLX and NLC Sensor Chips HTX Sensor Chips
LCP Chip and Liposome Capturing Kit
GLC Lipid Kit Ordering Information Related Literature
4
5
5
5
6
6
6
7
7
7
7
7
8
8
8
8
9
9
10
10
10
10
10
11
12
12
13
13
14
15
15
17
17
17
17
18
18
18
19
19
20
2121