Download - Bio-Rad

LCP Chip and Liposome Capturing Kit
Regeneration is accomplished by DNA dehybridization using the following
conditions. Injection 2 is optional because it is used to remove the remaining
lipid assemblies if the regeneration is incomplete with injection 1. For the first
injection, use a freshly prepared 8 M solution of urea in deionized water.
Table 10. LCP chip regeneration conditions.
Injection
1
2
Reagent
Orientation
Volume, µl
Flow Rate, µl/min
8 M urea*
20 mM CHAPS
Vertical
Vertical
150
150
30
30
* If urea is not available, inject deionized water.
GLC Lipid Kit
When using the GLC lipid kit, injecting lipid modification solution
(20 mM CHAPS) will regenerate the chip surface.
Table 11. GLC chip regeneration conditions.
Injection
1
Reagent
Orientation
Volume, µl
Flow Rate, µl/min
20 mM CHAPS
Vertical
150
30
19