Download TIGR MultipleExperimentViewer (MEV)

Transcript
Table of Contents
1. General Information............................................................... 6
2. TM4 Software Overview ........................................................ 7
3. Starting MultiExperiment Viewer ........................................ 8
4. Loading Expression Data ....................................................... 9
4.1.
Loading MeV (.mev) Format Files...................................................................... 9
4.2.
Loading TIGR Array Viewer (.tav) Files .......................................................... 10
4.3.
Loading Tab Delimited, Multiple Sample (.txt) Files (TDMS Format) ............ 11
4.4.
Loading Affymetrix Data (.txt, .TXT) Files ....................................................... 12
4.6.
Loading GenePix (.gpr) Data Files .................................................................. 13
4.7.
Loading Agilent Files........................................................................................ 14
4.13.
Initial View of the Loaded Data, Main Expression Image............................ 20
4.14.
Result Navigation Tree ................................................................................. 21
4.15.
The History Node and Log............................................................................ 21
5. Adjusting the Data................................................................ 23
5.1.
Adjustment / Filter Overview ............................................................................ 23
5.2.
Replicate Analysis............................................................................................. 24
5.3.
Data Transformations....................................................................................... 25
5.4.
Data Filters (Data Quality and Variance Based Filters) ................................. 27
5.5.
Data Source Selection....................................................................................... 32
6. Display Options..................................................................... 34
6.1.
Sample Annotation ............................................................................................ 34
6.2.
Selecting Gene Annotation................................................................................ 36
6.3.
Color Scheme Selection .................................................................................... 36
6.4.
Setting Color Scale Limits ................................................................................ 37
6.5.
Element Appearance ......................................................................................... 39
7. Viewer Descriptions.............................................................. 40
7.1.
Overview ........................................................................................................... 40
7.2.
Expression Images ............................................................................................ 40
7.3.
Expression Graphs............................................................................................ 43
7.4.
Centroid Graphs ............................................................................................... 45
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