Download TIGR MultipleExperimentViewer (MEV)
Transcript
Table of Contents 1. General Information............................................................... 6 2. TM4 Software Overview ........................................................ 7 3. Starting MultiExperiment Viewer ........................................ 8 4. Loading Expression Data ....................................................... 9 4.1. Loading MeV (.mev) Format Files...................................................................... 9 4.2. Loading TIGR Array Viewer (.tav) Files .......................................................... 10 4.3. Loading Tab Delimited, Multiple Sample (.txt) Files (TDMS Format) ............ 11 4.4. Loading Affymetrix Data (.txt, .TXT) Files ....................................................... 12 4.6. Loading GenePix (.gpr) Data Files .................................................................. 13 4.7. Loading Agilent Files........................................................................................ 14 4.13. Initial View of the Loaded Data, Main Expression Image............................ 20 4.14. Result Navigation Tree ................................................................................. 21 4.15. The History Node and Log............................................................................ 21 5. Adjusting the Data................................................................ 23 5.1. Adjustment / Filter Overview ............................................................................ 23 5.2. Replicate Analysis............................................................................................. 24 5.3. Data Transformations....................................................................................... 25 5.4. Data Filters (Data Quality and Variance Based Filters) ................................. 27 5.5. Data Source Selection....................................................................................... 32 6. Display Options..................................................................... 34 6.1. Sample Annotation ............................................................................................ 34 6.2. Selecting Gene Annotation................................................................................ 36 6.3. Color Scheme Selection .................................................................................... 36 6.4. Setting Color Scale Limits ................................................................................ 37 6.5. Element Appearance ......................................................................................... 39 7. Viewer Descriptions.............................................................. 40 7.1. Overview ........................................................................................................... 40 7.2. Expression Images ............................................................................................ 40 7.3. Expression Graphs............................................................................................ 43 7.4. Centroid Graphs ............................................................................................... 45 2